Mouse GLP-1(Glucagon Like Peptide 1) ELISA Kit (E-EL-M3108)

Name: Mouse GLP-1(Glucagon Like Peptide 1) ELISA Kit
Brand: Elabscience
SKU: E-EL-M3108
Price: 150.0 USD
Availability: InStock

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Mouse GLP-1(Glucagon Like Peptide 1) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	5.67 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GLP-1 in samples.No significant cross-reactivity or interference between Mouse GLP-1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.524	2.534	2.529	2.447
500	1.637	1.651	1.644	1.562
250	1.038	1.026	1.032	0.950
125	0.494	0.528	0.511	0.429
62.5	0.314	0.292	0.303	0.221
31.25	0.204	0.178	0.191	0.109
15.63	0.138	0.150	0.144	0.062
0	0.076	0.088	0.082	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.070	111.190	348.710	48.970	118.940	330.220
Standard deviation	2.730	5.440	14.400	2.600	5.220	13.010
CV（%）	5.460	4.890	4.130	5.300	4.390	3.940

Recovery

The recovery of Mouse GLP-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	88-99	94
Serum （n=8）	85-95	90
EDTA plasma （n=8）	94-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GLP-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	90-103	96-112
1:2	Average	92	97	104
1:4	Range	82-95	85-98	97-113
1:4	Average	89	92	103
1:8	Range	85-98	93-108	99-115
1:8	Average	89	100	105

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	98.63-112.96
Sample 2（n=16）	94.50-101.23

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GLP-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GLP-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GLP-1 . You can calculate the concentration of GLP-1 in the samples by comparing the OD of the samples to the standard curve.

Background

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1. GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels. GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.