Mouse GLP-1(Glucagon Like Peptide 1) ELISA Kit (E-EL-M3108)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GLP-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GLP-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GLP-1 . You can calculate the concentration of GLP-1 in the samples by comparing the OD of the samples to the standard curve.

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1. GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels. GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.