Mouse Klotho(Klotho) ELISA Kit (E-EL-M3051)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Klotho. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Klotho and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Klotho, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Klotho. You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve.

Klotho is a single-pass type I transmembrane protein identified as an anti-aging factor. Mice lacking the protein display a premature aging phenotype with arteriosclerosis, ectopic soft-tissue calcification, osteoporosis, skin atrophy, infertility and cognitive decline, whereas overexpression extends lifespan. It is synthesized mainly by renal proximal tubular cells and is also present in parathyroid gland, choroid plexus and brain; the extracellular domain can be shed by ADAM proteases into the circulation as soluble Klotho. Membrane Klotho functions as an obligate co-receptor for fibroblast growth factor 23 (FGF23), converting FGFR1c into a receptor with high affinity for the hormone and thereby mediating renal phosphate excretion, suppression of active vitamin D synthesis and mineral homeostasis that sustains bone and cardiovascular health. The shed soluble form acts independently as a humoral factor that inhibits insulin and IGF-1 signalling, antagonises Wnt and transforming growth factor beta (TGF-beta) activity, suppresses oxidative stress and inflammation and protects the vascular endothelium through nitric oxide production. Circulating Klotho declines with age and with chronic kidney disease, and low levels are associated with cardiovascular disease, diabetes, cognitive impairment and osteoporosis. Quantitative determination of Klotho therefore serves as a marker of renal function and biological aging in nephrology, ageing and metabolic research.