Mouse Lptn/XCL1(Lymphotactin) ELISA Kit (E-EL-M0763)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/XCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Lptn/XCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Lptn/XCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Lptn/XCL1. You can calculate the concentration of Mouse Lptn/XCL1 in the samples by comparing the OD of the samples to the standard curve.

XCL1, also known as lymphotactin, is an inflammatory chemokine secreted by T cells and natural killer (NK) cells. The XCL1-XCR1 axis has been shown to play an important role in the dendritic cell mediated cytotoxic immune response and in the formation of self-tolerance mechanisms through the development of regulatory T cells within the thymus. XCL1 also acts as a conformation-dependent inhibitor of HIV-1. Unlike other chemokines, XCL1 conformation interchanges between conserved chemokine folds and an unrelated dimeric structure under physiological conditions. The XCL1 monomer binds XCR1 with a high affinity, whereas the dimer form binds glycosaminoglycans (GAGs) with a high affinity, but fails to activate XCR1.