Mouse MCSFR (Macrophage colony-stimulating factor 1 receptor) ELISA Kit (E-EL-M3129)

Name: Mouse MCSFR (Macrophage colony-stimulating factor 1 receptor) ELISA Kit
Brand: Elabscience
SKU: E-EL-M3129
Price: 150.0 USD
Availability: InStock

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Mouse MCSFR (Macrophage colony-stimulating factor 1 receptor) ELISA Kit - 1

Size	Price	Qty
96T	$ 588.00	- +
48T	$ 470.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse MCSFR in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	2.234	2.160	2.197	2.117
2500	1.750	1.718	1.734	1.654
1250	0.945	0.953	0.949	0.869
625	0.534	0.542	0.538	0.458
312.5	0.297	0.307	0.302	0.222
156.25	0.177	0.185	0.181	0.101
78.13	0.137	0.127	0.132	0.052
0	0.076	0.084	0.080	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	232.120	662.330	2287.780	228.640	636.580	2118.440
Standard deviation	15.760	34.040	159.690	17.450	50.610	143.210
CV（%）	6.790	5.140	6.980	7.630	7.950	6.760

Recovery

The recovery of Mouse MCSFR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-103	97
EDTA plasma （n=8）	90-104	97
Cell culture media （n=8）	85-97	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse MCSFR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-111	97-112	92-103
1:2	Average	103	103	98
1:4	Range	95-106	98-112	88-100
1:4	Average	101	104	95
1:8	Range	98-112	99-114	90-104
1:8	Average	104	106	95

For the recovery experiment, the samples were spiked with standard solutions of a specific concentration and then serially diluted to 2-fold, 4-fold, 8-fold, and 16-fold dilutions, respectively. The average recovery rate was subsequently calculated.

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	105.97-110.05
Sample 2（n=16）	94.53-94.97

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCSFR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCSFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCSFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 5 nm. The OD value is proportional to the concentration of Mouse MCSFR. You can calculate the concentration of Mouse MCSFR in the samples by comparing the OD of the samples to the standard curve.

Background

MCSFR(Macrophage colony-stimulating factor 1 receptor), also known as CD115 and CSF1R, is a single-pass type I membrane tyrosine-protein kinase and a member of the platelet-derived growth factor receptor family. It acts as a cell-surface receptor for CSF1 (M-CSF) and IL34, playing essential roles in regulating survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, osteoclasts, and microglia. It mediates innate immunity, inflammatory processes, normal bone/tooth development, and mammary gland maturation during pregnancy. Ligand binding induces its homodimerization, phosphorylation and activation of downstream pathways (ERK1/2, JNK, AKT1), and promotes cancer cell invasion. It is expressed on monocytes/macrophages, dendritic cells and osteoclasts.