Mouse OXA(Orexin A) ELISA Kit (E-EL-M0860)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse OXA. During the reaction, Mouse OXA in the sample or standard competes with a fixed amount of Mouse OXA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse OXA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse OXA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Orexin A, also called hypocretin-1, is a 33-amino-acid neuropeptide generated from the prepro-orexin precursor encoded by the HCRT gene, which also yields orexin B. It contains two intramolecular disulfide bonds and carries N-terminal pyroglutamylation and C-terminal amidation. Orexin A is synthesized exclusively by a small cluster of neurons in the lateral and posterior hypothalamus that nevertheless projects widely throughout the brain and spinal cord. Orexins act through the G-protein-coupled receptors OX1R and OX2R, which couple mainly to Gq and raise intracellular calcium, and they are major regulators of arousal and vigilance state stability. They also stimulate feeding and energy expenditure, enhance locomotion and modulate autonomic, neuroendocrine, reward and stress-related functions, thereby integrating sleep-wake control with energy homeostasis. Orexin neurons receive metabolic, circadian and limbic inputs and excite monoaminergic and cholinergic arousal circuits. Loss of orexin neurons causes type 1 narcolepsy, in which cerebrospinal fluid orexin A is markedly reduced, and its measurement assists diagnosis and classification of hypersomnia. Orexin receptor antagonists are used clinically to promote sleep. This ELISA kit enables sensitive quantitative determination of orexin A in cerebrospinal fluid, serum and plasma, supporting sleep disorder, narcolepsy and feeding and energy research.