Mouse sIgA(Secretory Immunoglobulin A) ELISA Kit (E-EL-M1040)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse sIgA. You can calculate the concentration of Mouse sIgA in the samples by comparing the OD of the samples to the standard curve.

Secretory immunoglobulin A (sIgA) is the polymeric secreted form of IgA, typically composed of two to three IgA monomers joined by a J chain and associated with the secretory component. It is widely distributed in mucosal secretions including milk, saliva, tears, intestinal fluid and bronchoalveolar lavage fluid and is the principal effector molecule of mucosal humoral immunity. sIgA is produced by plasma cells in the lamina propria and submucosa, transported across epithelial cells by the polymeric immunoglobulin receptor and released into the lumen with the receptor-derived secretory component still attached, which protects the antibody from proteolytic degradation. Through immune exclusion it prevents microbial adhesion and colonization, agglutinates pathogens, neutralizes toxins and viruses and shapes the composition of the commensal microbiota. Neonates acquire sIgA passively through breast milk. sIgA levels reflect mucosal immune status, and their measurement is widely applied in research on mucosal immunity, the gut microbiota, breastfeeding and infection susceptibility. It is an important indicator of mucosal barrier defense, with declining levels signaling impaired barrier integrity. This ELISA kit enables quantitative determination of sIgA in serum, plasma, saliva, intestinal fluid and bronchoalveolar lavage fluid.