Mouse TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit (E-EL-M0051)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TGF-β1. You can calculate the concentration of Mouse TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Mouse transforming growth factor beta 1 (TGF-beta1) is a multifunctional cytokine that regulates cell growth, differentiation, immune function, and development and is the prototypical member of the TGF-beta superfamily. It is synthesized as a precursor whose C-terminal mature dimer is non-covalently associated with its latency-associated peptide (LAP), maintaining the cytokine in an inactive latent state that is deposited in the extracellular matrix. Latent TGF-beta1 is activated by integrin-mediated traction or by proteases and pH changes, after which it engages the TGFBR1 and TGFBR2 receptor serine/threonine kinases to phosphorylate SMAD2 and SMAD3, which partner with SMAD4 to regulate transcription. The pathway controls proliferation, apoptosis, epithelial-mesenchymal transition, and extracellular matrix synthesis. In immunity TGF-beta1 acts in a concentration-dependent manner to direct regulatory T cell or Th17 differentiation, while persistent excess signaling drives tissue fibrosis. Dysregulated TGF-beta1 is linked to fibrosis, cancer, atherosclerosis, and autoimmune disease, and within tumors it acts both as a suppressor of early lesions and as a promoter of invasion and metastasis. This ELISA kit enables quantitative determination of mouse TGF-beta1 in serum, plasma, and cell culture supernatant, supporting research on immune regulation, developmental biology, tissue repair, and fibrotic and malignant disease.