Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit (E-EL-M1153)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.

Tight junction protein 1 (TJP1), better known as zonula occludens-1 (ZO-1), is a peripheral membrane scaffold protein of about 220 kDa belonging to the membrane-associated guanylate kinase (MAGUK) family. Through PDZ, SH3 and guanylate kinase domains it links the transmembrane proteins claudin and occludin to the actin cytoskeleton and associates with ZO-2 and ZO-3 to assemble the junctional complex that seals the paracellular space. ZO-1 is expressed in intestinal and other epithelial sheets as well as in blood-brain-barrier endothelium. Beyond scaffolding, ZO-1 shuttles between the junction and the nucleus, where it influences expression of genes governing proliferation and epithelial differentiation, and it maintains apicobasal polarity and vectorial transport by coupling junction assembly to the cortical cytoskeleton. Its loss, redistribution or phosphorylation accompanies increased paracellular permeability, permitting solutes, inflammatory cells and pathogens to cross the epithelial or endothelial layer. This ELISA kit enables quantitative determination of ZO-1 in mouse tissue lysates and cell culture supernatants with high sensitivity and good reproducibility. The assay is applicable to barrier biology as well as to inflammation and tumour research, and is commonly used to evaluate tight junction integrity and barrier disruption in models of colitis, infection and metastasis. Changes in measurable ZO-1 therefore provide a practical read-out of epithelial barrier function.