NAD-Malic Enzyme (NAD-ME) Activity Colorimetric Assay Kit (E-BC-K556-M)
For research use only.
| Detection Principle |
Malic enzyme (ME) is widely present in both eukaryotes and prokaryotes and participates in various metabolic pathways. It mainly catalyzes the oxidation decarboxylation of malic acid to produce pyruvate and CO2, accompanied by the reduction of NAD (P) + to NAD (P) H. In mammalian cells, malic acid mainly includes ME1 in the cytoplasm that depends on NADP+, ME2 in the mitochondria that depends on NAD+, and ME3 in the mitochondria that depends on NADP+. The detection principle of this kit: Malic enzyme catalyzes the substrate reaction to generate NADH. There is a characteristic absorption peak at 340 nm. By measuring the increase rate of absorbance at 340 nm, the activity of NAD-ME can be characterized. |
| Sample Type | Animal tissue,Plant tissue,Cell,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader(340 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle,Mitochondria Function,Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 16.36 U/L |
| Detection Range | 16.36-157.10 U/L |
| Precision | inter-assay CV: 7.8%-9.9% | intra-assay CV: 2.9%-4.8% |
| Sample Volume | 5 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse muscle tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 5-10 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 4-10 |
| 10% Banana tissue homogenate | 2-3 |
| 10% Apple tissue homogenate | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 A549 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| Rat serum | 1 |
| Human serum | 1 |
| Mouse serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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