Nitrate Colorimetric Assay Kit (E-BC-K895-M)
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For research use only.
| Detection Principle | Nitric oxide (NO) is chemically active and is rapidly metabolized in the body to nitrite (NO2-) and nitrate (NO3-), and NO2- is further converted into NO3-. In this kit, NO3- was reduced to NO2- by reductant agent, and reacts with chromogenic agent to produce color substance. The nitrate content can be determined at 540 nm. |
| Sample Type | Serum,Plasma,Urine,Tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4), 2 mol/L NaOH |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.68 μmol/L |
| Detection Range | 0.68-100.00 μmol/L |
| Precision | inter-assay CV: 7.8-9.3% | intra-assay CV: 1.2-3.8% |
| Sample Volume | 100-150 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rat serum | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Mouse urine | 10-20 |
| 10% Mouse kidney tissue homogenate | 1-2 |
| 10% Mouse liver tissue homogenate | 1-2 |
| 10% Young garlic shoot tissue homogenate | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 293T cells | 1 |
| Cell culture supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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