Nitrite Reductase(NiR) Activity Colorimetric Assay Kit (E-BC-K863-M)
For research use only.
| Detection Principle | Nitrite reductase (NiR) in the sample can reduce NO2- to NO, so that the NO2- involved in the diazotization reaction to produce purplish red compounds is reduced, that is, the change of absorbance value at 540 nm can reflect the activity of the sample nitrite reductase. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (530-550 nm, optimum wavelength: 540 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.045 μmol/h/g |
| Detection Range | 0.045-9 μmol/h/g |
| Precision | inter-assay CV: 3.5-7.7% | intra-assay CV: 0.6-1.8% |
| Sample Volume | 200 μL(tissue homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Corn tissue homogenate | 1 |
| 10% Cabbages tissue homogenate | 1 |
| 10% Green pepper tissue homogenate | 1 |
| 10% Radish tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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