One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC) (E-CK-A420)

For research use only.
Detection Principle | When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry. |
Detection Method | Fluorometric method, Flow cytometry |
Sample Type | Cell samples |
Assay Time | 2.5-3 hours |
Detection Instrument | Flow Cytometer |
Dye Type | FITC |
Ex/Em | 490/530 |
Channel Set | FITC |
Other Reagents Required | PBS buffer (with 1%BSA) (PH7.2-7.4), PBS (with 1% Saponin) (pH7.2-7.6), 4% Polyformaldehyde (dissolved in PBS)(E-IR-R113), Deionized water. |
Storage | This product can be stored at -20°C for 12 months with shading light. |
Expiration date | 12 months |
Shipping | Ice bag |
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