QuicKey Pro Canine E2(Estradiol) ELISA Kit (E-OSEL-C0001)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 6.37 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Canine |
| Specificity | This kit recognizes Canine E2 in samples.No significant cross-reactivity or interference between Canine E2 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine E2 are added to the micro ELISA plate wells. Canine E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Canine E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
Estradiol, western drug name.Commonly used dosage forms are gels, patches, compound tablets, etc.For estrogen drugs.It is used for the following symptoms caused by estrogen deficiency caused by various reasons: hot flashes, sweating, sleep disorders, dizziness, genital atrophy, atrophic vaginitis, vaginal dryness, etc.
| Research Area | Cell Biology , Metabolism |
| Cat.No. | Product Name | Clone No. |
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