QuicKey Pro Human ApoA1 (Apolipoprotein A1) ELISA Kit (E-OSEL-H0072)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 1.88 ng/mL |
| Detection Range | 3.13-200 ng/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes ApoA1 in samples.No significant cross-reactivity or interference between ApoA1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 22 nm. The OD value is proportional to the concentration of ApoA1 . You can calculate the concentration of ApoA1 in the samples by comparing the OD of the samples to the standard curve.
Background
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
| Gene Alias | APOA1 |
| Gene ID | 335 |
| Uniport ID | P02647 |
| Protein Alias | APOA1 |
| Research Area | Cardiovascular , Signal Transduction , Metabolism , Neuroscience |
| Cat.No. | Product Name | Clone No. |
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