QuicKey Pro Human CD147 (Extracellular Matrix Metalloproteinase Inducer) ELISA Kit (E-OSEL-H0050)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD147. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CD147 are added to the micro ELISA plate wells. Human CD147 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CD147.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD147. The concentration of Human CD147 in the samples is then determined by comparing the OD of the samples to the standard curve.

BSG,also named as 5F7,Basigin,EMMPRIN,TCSF and CD147,plays pivotal roles in spermatogenesis,embryo implantation,neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1,SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA,inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix,plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers.