QuicKey Pro Human CTSB (Cathepsin B) ELISA Kit (E-OSEL-H0051)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSB. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CTSB are added to the micro ELISA plate wells. Human CTSB in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CTSB.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSB. The concentration of Human CTSB in the samples is then determined by comparing the OD of the samples to the standard curve.

The protein encoded by CTSB gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene.