QuicKey Pro Human CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit (E-OSEL-H0047)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCL1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CXCL1 are added to the micro ELISA plate wells. Human CXCL1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CXCL1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CXCL1. The concentration of Human CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

CXCL1 (C-X-C Motif Chemokine Ligand 1) is a Protein Coding gene. Diseases associated with CXCL1 include Melanoma and Bacterial Meningitis. Among its related pathways are Peptide ligand-binding receptors and Chemokine Superfamily Pathway: Human/Mouse Ligand-Receptor Interactions. GO annotations related to this gene include receptor binding and chemokine activity. An important paralog of this gene is CXCL2.This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.