QuicKey Pro Human Cys-C (Cystatin C) ELISA Kit (E-OSEL-H0021)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys-C.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis.