QuicKey Pro Human DKK1 (Dickkopf Related Protein 1) ELISA Kit (E-OSEL-H0059)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 0.09 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes DKK1 in samples.No significant cross-reactivity or interference between DKK1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human DKK1 are added to the micro ELISA plate wells. Human DKK1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human DKK1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DKK1. The concentration of Human DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
| Gene Alias | DKK1 |
| Gene ID | 22943 |
| Uniport ID | O94907 |
| Protein Alias | DKK1 |
| Research Area | Cancer , Cardiovascular , Developmental Biology , Immunology |
| Cat.No. | Product Name | Clone No. |
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