QuicKey Pro Human E-Cad(E-Cadherin) ELISA Kit (E-OSEL-H0017)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 46.88 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human E-Cad in samples. No significant cross-reactivity or interference between Human E-Cad and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E-Cad are added to the micro ELISA plate wells. Human E-Cad in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human E-Cad.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human E-Cad. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
| Gene ID | 999 |
| Uniport ID | P12830 |
| Research Area | Cancer , Developmental Biology , Signal Transduction |
| Cat.No. | Product Name | Clone No. |
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