QuicKey Pro Human FE(Ferritin) ELISA Kit (E-OSEL-H0003)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 24.31 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human FE in samples.No significant cross-reactivity or interference between Human FE and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FE are added to the micro ELISA plate wells. Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FE.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human FE. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
| Gene ID | 2495;2512 |
| Uniport ID | P02792 P02794 |
| Research Area | Cancer , Cardiovascular , Metabolism , Neuroscience , Signal Transduction |
| Cat.No. | Product Name | Clone No. |
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