QuicKey Pro Human HCG (Chorionic Gonadotropin) ELISA Kit (E-OSEL-H0026)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 4.69 mIU/mL |
| Detection Range | 7.81-500 mIU/mL |
| Sample Volume | 50 μL |
| Manual Operation Time | 30 min |
| Total Assay Time | 1 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes HCG in samples.No significant cross-reactivity or interference between HCG and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HCG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human HCG are added to the micro ELISA plate wells. Human HCG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human HCG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HCG. The concentration of Human HCG in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
CGA shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH,lutropin/luteinizing hormone/LH,follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
| Gene Alias | CGA |
| Gene ID | 1081 |
| Uniport ID | P01215(CGA)P0DN86(CGβ) |
| Protein Alias | CGA |
| Research Area | Cancer , Developmental Biology , Metabolism , Signal Transduction |
| Cat.No. | Product Name | Clone No. |
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