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QuicKey Pro Human IgG (Immunoglobulin G) ELISA Kit (E-OSEL-H0023)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 520.00
48T $ 416.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 0.47 ng/mL
Detection Range 0.78-50 ng/mL
Sample Volume 50 μL
Manual Operation Time 30 min
Total Assay Time 1 h 30 min
Reacitivity Human
Specificity This kit recognizes IgG in samples.No significant cross-reactivity or interference between IgG and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IgG are added to the micro ELISA plate wells. Human IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IgG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgG. The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
IgG is a monomeric immunoglobulin composed of two identical heavy chains and two identical light chains linked together by inter-chain disulfide bonds. There are four IgG subclasses (IgG1, IgG2, IgG3, and IgG4) in humans named in order of decreasing abundance. They differ in the size of the hinge region and the number and arrangements of the interchain disulfide bonds linking their heavy chains. Of the 5 immunoglobulin isotypes, IgG is most abundant in human serum. IgG is synthesized and secreted by plasma B cells and is involved in the secondary immune response. The measurement of IgG can be a diagnostic tool for certain conditions such as autoimmune diseases, gastrointestinal conditions or immunoglobulin-deficiency disorders.
Research Area Immunology , Cell Biology
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