Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reactivity	Human
Specificity	This kit recognizes LDLR in samples.No significant cross-reactivity or interference between LDLR and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LDLR. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LDLR are added to the micro ELISA plate wells. Human LDLR in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LDLR.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LDLR. The concentration of Human LDLR in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits,which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Gene Alias	LDLR
Gene ID	3949
Uniport ID	P01130
Protein Alias	LDLR
Research Area	Cell Biology , Neuroscience

QuicKey Pro Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 520.00	- +
48T	$ 416.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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QuicKey Pro Human LDLR (Low Density Lipoprotein Receptor) ELISA Kit (E-OSEL-H0040)

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