QuicKey Pro Human OPG (Osteoprotegerin) ELISA Kit (E-OSEL-H0034)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human OPG are added to the micro ELISA plate wells. Human OPG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human OPG.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPG. The concentration of Human OPG in the samples is then determined by comparing the OD of the samples to the standard curve.

The protein encoded by TNFRSF11B gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.