QuicKey Pro Human PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit (E-OSEL-H0033)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PⅢNP are added to the micro ELISA plate wells. Human PⅢNP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PⅢNP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. The concentration of Human PⅢNP in the samples is then determined by comparing the OD of the samples to the standard curve.

COL3A1 gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.