QuicKey Pro Human SELE (E-Selectin) ELISA Kit (E-OSEL-H0045)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SELE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human SELE are added to the micro ELISA plate wells. Human SELE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human SELE.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SELE. The concentration of Human SELE in the samples is then determined by comparing the OD of the samples to the standard curve.

The protein encoded by SELE gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.