QuicKey Pro Human VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit (E-OSEL-H0049)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human VEGFR-2 are added to the micro ELISA plate wells. Human VEGFR-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human VEGFR-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR-2. The concentration of Human VEGFR-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.