QuicKey Pro Mouse CXCL7 (Thromboglobulin, Beta) ELISA Kit (E-OSEL-M0022)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL7. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse CXCL7 are added to the micro ELISA plate wells. Mouse CXCL7 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse CXCL7.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CXCL7. The concentration of Mouse CXCL7 in the samples is then determined by comparing the OD of the samples to the standard curve.

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.