QuicKey Pro Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit (E-OSEL-M0021)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGFR. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse EGFR are added to the micro ELISA plate wells. Mouse EGFR in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse EGFR.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EGFR. The concentration of Mouse EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.

Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.