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QuicKey Pro Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit (E-OSEL-M0021)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 520.00
48T $ 416.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 0.09 ng/mL
Detection Range 0.16-10 ng/mL
Sample Volume 50 μL
Manual Operation Time 30 min
Total Assay Time 1 h 30 min
Reacitivity Mouse
Specificity This kit recognizes EGFR in samples.No significant cross-reactivity or interference between EGFR and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGFR. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse EGFR are added to the micro ELISA plate wells. Mouse EGFR in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse EGFR.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse EGFR. The concentration of Mouse EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Epidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.
Gene Alias Egfr
Gene ID 13649
Uniport ID Q01279
Protein Alias Egfr
Research Area Cell Biology
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