QuicKey Pro Rat ACE2 (Angiotensin Ⅰ Converting Enzyme 2) ELISA Kit

Size	Price	Qty
96T	$ 520.00
48T	$ 416.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.23 ng/mL
Detection Range	0.39-25 ng/mL
Sample Volume	50 μL
Manual Operation Time	30 min
Total Assay Time	1 h 30 min
Reacitivity	Rat
Specificity	This kit recognizes ACE2 in samples.No significant cross-reactivity or interference between ACE2 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ACE2 are added to the micro ELISA plate wells. Rat ACE2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ACE2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE2. The concentration of Rat ACE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

The protein encoded by Ace2 gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9; and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function; as well as fertility. In addition; the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

Gene Alias	Ace2
Gene ID	302668
Uniport ID	Q5EGZ1
Protein Alias	Ace2
Research Area	Cancer , Cell Biology , Cardiovascular

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QuicKey Pro Rat ACE2 (Angiotensin Ⅰ Converting Enzyme 2) ELISA Kit (E-OSEL-R0012)

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