Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	1.87 ng/mL
Detection Range	4.69-300 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat Cort in samples.No significant cross-reactivity or interference between Rat Cort and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma, Urine, Saliva
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Cort are added to the micro ELISA plate wells. Rat Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Research Area

Cell Biology

QuicKey Pro Rat CORT(Corticosterone) ELISA Kit - procedures

Other Clones

{{antibodyDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Other Formats

{{formatDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

IF：{{item.impact}}

Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}
Reactivity：{{item.species}}

Sample Type：{{item.organization}}

Previous
{{ page }}
Next

Species

{{relatedElisaPage.numTotal}} Results

All

Chicken

Mouse

Rat

Rat/Chicken

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Previous
{{ page }}
Next

Size	Price	Qty
96T	$ 520.00	- +
48T	$ 416.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

All categories

QuicKey Pro Rat CORT(Corticosterone) ELISA Kit (E-OSEL-R0002)

Other Clones

Other Formats

Species