QuicKey Pro Rat Pg(Progesterone) ELISA Kit (E-OSEL-R0007)
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For research use only.
Product Summary
Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.
| Sensitivity | 7.01 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 1 h 30 min |
| Reactivity | Rat |
| Specificity | This kit recognizes Rat Pg in samples.No significant cross-reactivity or interference between Rat Pg and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 6 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Background
Progesterone (Progesterone), also known as progesterone, progesterone, progesterone, lutein, etc., is a steroid progesterone that is one of the essential hormones to maintain the normal reproductive function of female mammals. It is mainly produced and secreted by the lutein. Progesterone acts on a variety of tissues and organs, such as the brain, breasts, uterus, ovaries, and cervix. Progesterone has a complex physiological effect in the body, acting on different cells of the same tissue will also produce different effects, under physiological and pathological conditions, the effect of progesterone is also very different. For example, in breast cancer and uterine leiomyoma, progesterone promotes tumor development and progression. However, in endometrial cancer, progesterone has shown a certain anti-cancer effect. The specific reasons for this phenomenon are not clear at present. One possibility is that some specific factors, receptor expression, paracrine and autocrine processes in the special environment of different tissues affect the effect of progesterone.
| Research Area | Signal Transduction |
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