QuicKey Pro Rat SELP (P-Selectin) ELISA Kit (E-OSEL-R0011)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat SELP are added to the micro ELISA plate wells. Rat SELP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat SELP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SELP. The concentration of Rat SELP in the samples is then determined by comparing the OD of the samples to the standard curve.

CD62P is a 140 kD type I transmembrane glycoprotein, also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.