QuicKey Pro Rat VCAM-1/CD106 (Vascular Cell Adhesion Molecule 1) ELISA Kit (E-OSEL-R0014)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat VCAM-1/CD106 are added to the micro ELISA plate wells. Rat VCAM-1/CD106 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat VCAM-1/CD106.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. The concentration of Rat VCAM-1/CD106 in the samples is then determined by comparing the OD of the samples to the standard curve.

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses,binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.