Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.06 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reactivity	Sheep
Specificity	This kit recognizes Sheep Pg in samples.No significant cross-reactivity or interference between Sheep Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep Pg are added to the micro ELISA plate wells. Sheep Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Progesterone (progesterone), also known as progesterone, progesterone, is the main progesterone secreted by the ovary with biological activity. Its chemical formula is C21H30O2, the main role is to protect the female endometrium, during female pregnancy, progesterone hormone can provide support and protection for the early growth and development of the fetus, and can play a certain calming effect on the uterus.

Research Area

Signal Transduction