Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit (E-EL-R1430)

Name: Rat Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit
Brand: Elabscience
SKU: E-EL-R1430
Price: 150.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat Ang-Ⅱ in samples.No significant cross-reactivity or interference between Rat Ang-Ⅱ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	0.189	0.197	0.193	-
500	0.309	0.321	0.315	-
250	0.510	0.484	0.497	-
125	0.837	0.817	0.827	-
62.5	1.193	1.181	1.187	-
31.25	1.534	1.578	1.556	-
15.63	1.832	1.854	1.843	-
0	2.361	2.257	2.309	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	49.290	86.480	411.450	53.980	89.040	435.960
Standard deviation	2.960	4.270	19.340	3.990	6.030	37.580
CV（%）	6.000	4.940	4.700	7.400	6.770	8.620

Recovery

The recovery of Rat Ang-Ⅱ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-100	94
EDTA plasma （n=8）	90-104	96
Cell culture media （n=8）	84-96	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat Ang-Ⅱ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-107	92-107	85-97
1:2	Average	100	98	90
1:4	Range	96-108	91-103	84-95
1:4	Average	102	97	90
1:8	Range	98-113	90-102	86-97
1:8	Average	104	96	92

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	95.98-105.33
Sample 2（n=16）	106.23-106.78

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-Ⅱ. During the reaction, Rat Ang-Ⅱ in the sample or standard competes with a fixed amount of Rat Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

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