Rat BNP(Brain Natriuretic Peptide) ELISA Kit (E-EL-R0126)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. During the reaction, Rat BNP in the sample or standard competes with a fixed amount of Rat BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

B-type natriuretic peptide (BNP), also known as brain natriuretic peptide, is a nonglycosylated peptide hormone of the natriuretic peptide family, characterized by a conserved intramolecular disulfide ring, and is produced predominantly by ventricular cardiomyocytes although it was first identified in porcine brain. Transcription and release rise markedly when myocardial wall stress increases with pressure overload or volume expansion, and the precursor preproBNP is cleaved to yield the inactive amino-terminal fragment NT-proBNP together with biologically active BNP. BNP serves to unload the volume-overloaded or failing heart. Binding to the guanylyl cyclase-coupled receptor NPR1 raises intracellular cGMP, producing vasodilation and natriuresis that lower cardiac preload and afterload, while antagonizing the renin-angiotensin-aldosterone system, sympathetic outflow and maladaptive hypertrophy and fibrosis. The N-terminal fragment NT-proBNP lacks biological activity but is cleared more slowly and is therefore a more stable circulating analyte. Plasma BNP and NT-proBNP are established biomarkers in cardiovascular medicine, used for heart failure diagnosis, risk stratification and treatment monitoring. This ELISA kit enables quantitative determination of rat BNP in serum and plasma, supporting evaluation of cardiac loading, ventricular remodeling and responses to pathological stress or cardiovascular therapy.