Rat C5a(Complement Component 5a) ELISA Kit (E-EL-R3011)

Name: Rat C5a(Complement Component 5a) ELISA Kit
Brand: Elabscience
SKU: E-EL-R3011
Price: 150.0 USD
Availability: InStock

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Rat C5a(Complement Component 5a) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 ng/mL
Detection Range	15.63-1000 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat C5a in samples.No significant cross-reactivity or interference between Rat C5a and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.151	2.155	2.153	2.060
500	1.462	1.466	1.464	1.371
250	0.813	0.857	0.835	0.742
125	0.446	0.420	0.433	0.340
62.5	0.295	0.283	0.289	0.196
31.25	0.180	0.188	0.184	0.091
15.63	0.143	0.135	0.139	0.046
0	0.096	0.090	0.093	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	46.490	103.860	411.040	46.690	107.840	391.710
Standard deviation	3.040	6.240	27.010	3.970	9.510	26.560
CV（%）	6.540	6.010	6.570	8.510	8.820	6.780

Recovery

The recovery of Rat C5a was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-103	97
EDTA plasma （n=8）	92-104	99
Cell culture media （n=8）	87-97	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat C5a and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-104	99-110	89-101
1:2	Average	98	105	95
1:4	Range	96-108	86-102	84-100
1:4	Average	101	94	92
1:8	Range	88-101	101-109	98-110
1:8	Average	94	105	103

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	111.16-111.43
Sample 2（n=16）	87.12-107.71

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C5a. You can calculate the concentration of Rat C5a in the samples by comparing the OD of the samples to the standard curve.

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