Rat CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit (E-EL-R1327)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CKMB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CKMB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CKMB. You can calculate the concentration of Rat CKMB in the samples by comparing the OD of the samples to the standard curve.

Creatine kinase MB isoenzyme (CK-MB) is a cytosolic enzyme formed as a heterodimer of muscle (M) and brain (B) subunits. It catalyzes the reversible transfer of phosphate between ATP and creatine to generate phosphocreatine, thereby providing a rapidly mobilizable high-energy phosphate reserve for tissues with high and fluctuating energy demand such as the myocardium, where it accounts for roughly 15-30% of total creatine kinase activity. Physiologically, CK-MB regenerates ATP from phosphocreatine when energy demand rises, sustaining myocardial contraction and ion pump function. When cardiomyocytes are injured and membrane integrity is lost, cytosolic CK-MB escapes into the circulation in proportion to the extent of necrosis; after acute myocardial infarction its level rises within a few hours, peaks at about one day and returns to baseline within two to three days. CK-MB was long a cornerstone of myocardial infarction diagnosis and remains useful for detecting reinfarction, and it is also elevated in myocarditis, after cardiac surgery and in severe skeletal muscle injury. This ELISA kit enables quantitative determination of rat CK-MB in serum and plasma, and it is used in myocardial injury and ischemia-reperfusion studies as a classic indicator of myocardial necrosis.