Rat D2D(D-Dimer) ELISA Kit (E-EL-R0317)
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For research use only.
Product Summary
| Sensitivity | 46.88 ng/mL |
| Detection Range | 78.13-5000 ng/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 2 h 30 min |
| Reactivity | Rat |
| Specificity | This kit recognizes Rat D2D in samples.No significant cross-reactivity or interference between Rat D2D and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat D2D. During the reaction, Rat D2D in the sample or standard competes with a fixed amount of Rat D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat D2D. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat D2D in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Background
A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process.
| Research Area | Cardiovascular |
| Cat.No. | Product Name | Clone No. |
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