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Rat IgG2b(Immunoglobulin G2b) ELISA Kit (E-EL-R3062)

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All Size Price Qty
96T $ 609.00
48T $ 487.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 65.63 pg/mL
Detection Range 109.38-7000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Rat
Specificity This kit recognizes Rat IgG2b in samples. No significant cross-reactivity or interference between Rat IgG2b and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG2b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG2b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG2b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgG2b. You can calculate the concentration of Rat IgG2b in the samples by comparing the OD of the samples to the standard curve.
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.Immunoglobulin G (IgG) is an antibody produced and released by plasma B cells. IgG is the main type of antibody in blood and extracellular fluid that can control infection in human tissues. Antibodies protect the body from infection by binding to a variety of pathogens, such as viruses, bacteria and fungi. In humans, IgG has four subtypes - IgG1, IgG2, IgG3, and IgG4. In mice and rats, IgG also has four subtypes - IgG1, IgG2a, IgG2b, and IgG3. In some cases, autoimmune hepatitis may present with certain symptoms, and IgG testing is a diagnostic tool. Clinically, the determination of IgG levels is generally considered to be an indication of the immune status of the body against a particular pathogen.
Research Area Immunology
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