Rat IgG2c(Immunoglobulin G2c) ELISA Kit (E-EL-R3063)

Name: Rat IgG2c(Immunoglobulin G2c) ELISA Kit
Brand: Elabscience
SKU: E-EL-R3063
Price: 150.0 USD
Availability: InStock

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Rat IgG2c(Immunoglobulin G2c) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	3.75 pg/mL
Detection Range	6.25-400 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat IgG2c in samples. No significant cross-reactivity or interference between Rat IgG2c and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
400	2.318	2.390	2.354	2.265
200	1.559	1.601	1.580	1.491
100	0.917	0.881	0.899	0.810
50	0.513	0.499	0.506	0.417
25	0.323	0.331	0.327	0.238
12.5	0.203	0.193	0.198	0.109
6.25	0.141	0.147	0.144	0.055
0	0.088	0.090	0.089	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	21.610	55.020	187.170	23.310	51.510	184.220
Standard deviation	1.040	3.450	9.810	1.650	3.900	12.340
CV（%）	4.790	6.270	5.240	7.080	7.580	6.700

Recovery

The recovery of Rat IgG2c was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-100	94
EDTA plasma （n=8）	93-105	100
Cell culture media （n=8）	87-103	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat IgG2c and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-107	89-102	90-104
1:2	Average	100	95	98
1:4	Range	93-107	85-100	90-105
1:4	Average	100	91	97
1:8	Range	92-107	91-101	93-106
1:8	Average	98	96	99

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	95.40-103.03
Sample 2（n=16）	92.17-94.06

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG2c. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG2c and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG2c, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgG2c. You can calculate the concentration of Rat IgG2c in the samples by comparing the OD of the samples to the standard curve.

Background

IgG2 is mainly used to neutralize antigens or block receptor ligand binding, and its CDC and ADCC effects are very weak, and only EGFR antibodies were marketed based on IGG2 in the early days. However, with the rise of immune checkpoint research, more and more IGG2-based drugs have entered the clinic and entered the market. Although IgG2 has weak binding with C1q, it can still trigger CDC effect when the antigen or antibody solubility is high, and IgG2 is the only subtype that can bind FC-γriia (CD32a). Moreover, this binding can be regulated by single nucleotide polymorphisms (SNPS), which can affect the functional activity of IgG2.

Research Area

Immunology