elabscienceelabscience
My Cart
Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Apoptosis and Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification Kits
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • Ⅰ
  • Ⅱ
  • Ⅲ
  • Ⅳ
  • Ⅴ
  • Ⅵ
  • Ⅶ
  • Ⅷ
  • Ⅸ
  • Ⅹ
  • Ⅺ
  • Ⅻ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑
|Register My Cart 1

Rat OC/BGP (Osteocalcin) ELISA Kit (E-EL-R3070)

Copy the product info.
Citations ({{ citationsPage.numTotal }}) Manual MSDS
Resources
All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
Bulk request >>
Add to cart

For research use only.

Product Summary
Sensitivity 18.75 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Rat
Specificity This kit recognizes OC/BGP in samples.No significant cross-reactivity or interference between OC/BGP and analogues was observed.
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to OC/BGP . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain OC/BGP , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of OC/BGP . You can calculate the concentration of OC/BGP in the samples by comparing the OD of the samples to the standard curve.
The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein: it acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization. The carboxylated form binds strongly to apatite and calcium.The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.
Uniport ID P04640
Research Area Cancer , Signal Transduction , Stem Cells
Other Clones

{{antibodyDetailsPage.numTotal}} Results

Other Formats

{{formatDetailsPage.numTotal}} Results

  • IF：{{item.impact}}

    Journal：{{item.journal}} ({{item.year}})

    DOI：{{item.doi}}

    Reactivity：{{item.species}}

    Sample Type：{{item.organization}}

Species

{{relatedElisaPage.numTotal}} Results

  • Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Our website uses cookies to enhance user experience. Read our Cookie Policy to find out more.