Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit (E-EL-R0951)

Name: Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit
Brand: Elabscience
SKU: E-EL-R0951
Price: 150.0 USD
Availability: InStock

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Rat sC5b-9(Soluble Terminal Complement Complex C5b-9) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.1 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat sC5b-9 in samples. No significant cross-reactivity or interference between Rat sC5b-9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	2.316	2.276	2.296	2.212
5	1.598	1.584	1.591	1.507
2.5	0.953	0.943	0.948	0.864
1.25	0.498	0.518	0.508	0.424
0.63	0.314	0.330	0.322	0.238
0.32	0.200	0.188	0.194	0.110
0.16	0.143	0.143	0.143	0.059
0	0.087	0.081	0.084	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.550	1.470	4.710	0.520	1.340	4.470
Standard deviation	0.020	0.090	0.320	0.050	0.080	0.290
CV（%）	4.150	5.850	6.900	8.890	6.310	6.380

Recovery

The recovery of Rat sC5b-9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	86-98	93
Serum （n=8）	91-104	96
EDTA plasma （n=8）	94-110	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat sC5b-9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-100	89-103	91-105
1:2	Average	93	96	97
1:4	Range	88-100	81-93	90-106
1:4	Average	93	86	98
1:8	Range	83-96	79-94	85-99
1:8	Average	90	86	92

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	97.30-112.66
Sample 2（n=16）	103.81-105.87

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sC5b-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sC5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sC5b-9. You can calculate the concentration of Rat sC5b-9 in the samples by comparing the OD of the samples to the standard curve.

Background

The terminal complement complex (TCC, SC5b-9) is the result of a divergent pathway that activates the complement system by the classical, bypass or MBL pathways. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates irreversible target cell membrane damage associated with complement activation. The C5b-9 complex formed in the absence of target membranes binds to naturally occurring regulatory serum proteins, such as S proteins, to form steady state SC5b-9.The status of the terminal complement pathway in a sample is indicated by measuring the concentration of TCC. The membrane attack complex, at abnormally elevated concentrations, exhibits cytotoxicity.

Research Area

CellBiology , Metabolism , Immunology , Signaltransduction