Rat TXB2(Thromboxane B2) ELISA Kit (E-EL-R0965)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat TXB2. During the reaction, Rat TXB2 in the sample or standard competes with a fixed amount of Rat TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat TXB2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Thromboxane A2 (TXA2), is an eicosanoid involved in platelet aggregation, vasoconstriction, and reproductive functions. However, as TXA2 is rapidly degraded in vivo, TXA2 production is typically examined by assaying the more stable Thromboxane B2 (TXB2) or 11-dehydro-TXB2 (11-d-TXB2) derivatives. Levels of these metabolites are helpful markers in the study of diseases including liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and others.