TXA2(Thromboxane A2) ELISA Kit (E-EL-0057)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal TXA2. During the reaction, Universal TXA2 in the sample or standard competes with a fixed amount of Universal TXA2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal TXA2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal TXA2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Thromboxane A2 (TxA2) is in the family of lipids known as eicosanoids, which are metabolites of arachidonic acid generated by the sequential action of 3 enzymes—phospholipase A2, COX-1无COX-2 and TxA2 Synthase (TXAS). TxA2 was originally described as being released from platelets and is now known to be released by various other cells, including macrophages, neutrophils, and endothelial cells. Named after its role in thrombosis, TxA2 has prothrombotic properties, as it stimulates the activation of platelets and platelet aggregation. TxA2 is also a known vasoconstrictor and gets activated during times of tissue injury and inflammation. While prostaglandin counterbalances its thrombotic and vasoconstrictor properties, prostacyclin (PGI2) has various physiological and pathological situations where this balance becomes dysregulated. Increased activity of TxA2 may play a role in the pathogenesis of myocardial infarction, stroke, atherosclerosis, and bronchial asthma. Increased action of TxA2 also has implications in pulmonary hypertension, kidney injury, hepatic injury, allergies, angiogenesis, and metastasis of cancer cells.