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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.94 ng/mL
Detection Range 1.56-100 ng/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Universal
Specificity This kit recognizes Universal VD3 in samples.No significant cross-reactivity or interference between Universal VD3 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal VD3. During the reaction, Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Vitamin D3 is also known as cholecalciferol, is a kind of vitamin D, cholesterol dehydrogenation after the generation of 7-dehydrogenated cholesterol by ultraviolet irradiation can form cholecalciferol, so that the vitamin D of cholecalciferol is 7-dehydrogenated cholesterol.
Research Area Signal Transduction , Metabolism
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