Flow Cytometry
Flow Cytometry FAQs
In order to provide better customer support and address customers' questions about FCM antibodies, we have compiled and published answers to the frequently-asked questions (FAQs) about FCM antibodies, which will be continuously updated.
-
It is not recommended, as the cell viability deteriorates after thawing, with a large number of cells dying, which significantly affects subsequent experiments.
-
The homogeneity of the magnetic beads is easily affected by the buffer. Washing the beads with sorting buffer helps maintain good homogeneity in that buffer. Experiments have shown that adding unwashed beads directly to cells can affect the purity and yield of the final sorted cells.
-
The principle of the kit is indeed that biotinylated antibodies bind to cells, followed by binding of the biotinylated antibodies to streptavidin-labeled magnetic beads. However, we do not recommend mixing components from other manufacturers, as incompatibility may occur.
-
This product cannot be used for column-based sorting; it can only be used for tube-based sorting with a magnetic stand.
-
The human CD3+T cell isolation kit uses PBMC as the sample, not directly peripheral blood. One assay is used to sort 1×10^7 PBMC cells, which corresponds to about 10 mL of human blood.
