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One-step TUNEL Assay Kit

Elabscience® offers you a highly sensitive, fast and simple TUNEL apoptosis assay kit with one-step dewaxing solution, which can better assist in apoptosis research on diseases related to cell function and R&D of related drugs. TUNEL apoptosis assay kits detect the DNA breaks formed when DNA fragmentation occurs in the last phase of apoptosis, monitor and quantify apoptosis.

One-step TUNEL assay kit ( FITC, AF488, AF594, AF647, AF555) is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, frozen section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. The test results can be directly observed through a fluorescence microscope.

Sensitive Quantitative Detection of Early Apoptosis,Click Here To Check Annexin V Apoptosis Detection Kits.

Activated Caspase can Induce Apoptosis, Caspase Expressed in The Early and Late Stages of Apoptos is,Click Here to Check Caspase Assay Kits.

Elabscience® One-step TUNEL Assay Products

Product Name Ex/Em Price
One-step TUNEL In Situ Apoptosis Kit (Green, FITC) 20 Assays/ 50 Assays/ 100 Assays $150 / $220 / $420
One-step TUNEL In Situ Apoptosis Kit (Green, AF488) 20 Assays/ 50 Assays/ 100 Assays $150 / $220 / $420
One-step TUNEL In Situ Apoptosis Kit (Red, AF594) 20 Assays/ 50 Assays/ 100 Assays $150 / $220 / $420
One-step TUNEL In Situ Apoptosis Kit (Red, AF647) 20 Assays/ 50 Assays/ 100 Assays $150 / $220 / $420
One-step TUNEL In Situ Apoptosis Kit (Red, AF555) 20 Assays/ 50 Assays/ 100 Assays $150 / $220 / $420

How to choose an appropriate TUNEL assay kit?

View One-step TUNEL (Cell Slide) Operation Guide

View One-step TUNEL (Paraffin Section) Operation Guide

Advantages

One step dewaxing saves about 2h, safe, non-toxic and environment friendly.

  • Company R
    Elabscience

    Traditional dewaxing methods
    Green fluorescence
    (Illumination value: 10, exposure time: 100 ms)

  • Traditional dewaxing method
    One-step dewaxing method

    Comparison of traditional and one-step dewaxing
    Green fluorescence
    (Illumination value: 10, exposure time: 30 ms)

Detection Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. After extraction and loading for electrophoresis, a DNA ladder of 180-200 bp can be found in such DNA samples, which is a specific event during cell apoptosis. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with labeled dUTP, which can be detected with fluorescence microscopy or flow cytometry.

Other Cell Related Assay Kits

Annexin V Apoptosis Detection KitsAnnexin V Apoptosis Detection Kits
Caspase Assay kitsCaspase Assay Kits
Cell Cycle Assay kitCell Cycle Assay Kit
Mitochondrial Membrane Potential Assay Kit (with JC-1)Mitochondrial Membrane Potential Assay Kit (with JC-1)
Cell Proliferation and Toxicity Detection kitsCell Proliferation and Toxicity Detection Kits
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